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ZHOU Hongbo, CHEN Zhuchang
《结构与土木工程前沿(英文)》 2007年 第1卷 第4期 页码 458-463 doi: 10.1007/s11709-007-0062-7
Performance of seven highway construction contracting methods analyzed by project size
Yuanxin ZHANG, Abdol CHINI, R. Edward MINCHIN Jr., Lourdes PTSCHELINZEW, Dev SHAH
《工程管理前沿(英文)》 2018年 第5卷 第2期 页码 240-250 doi: 10.15302/J-FEM-2018040
关键词: alternative contracting methods time cost performance evaluation
Jian GONG
《工程管理前沿(英文)》 2017年 第4卷 第1期 页码 106-109 doi: 10.15302/J-FEM-2017001
关键词: super-high-rise building green building integral steel platform formwork system large volume concrete concrete pumped for super-high building deep and large foundation pit combined anterograde and retrograde methods digitalized construction technology
A review of optimization modeling and solution methods in renewable energy systems
《工程管理前沿(英文)》 页码 640-671 doi: 10.1007/s42524-023-0271-3
关键词: renewable energy system bibliometrics mathematical programming optimization models solution methods
Shen LIU, Shengzhe SHANG, Xuezhen YANG, Huihua ZHANG, Dan LU, Ning LI
《农业科学与工程前沿(英文)》 2018年 第5卷 第3期 页码 382-389 doi: 10.15302/J-FASE-2018211
The mammary gland provides a novel method for producing recombinant proteins in milk of transgenic animals. A key component in the technology is the construction of an efficient milk expression vector. Here, we established a simple method to construct a milk expression vector, by a combination of homologous recombination and digestion-ligation. Our methodology is expected to have the advantages of both plasmid and bacterial artificial chromosome (BAC) vectors. The BAC of mouse whey acidic protein gene (mWAP) was modified twice by homologous recombination to produce a universal expression vector, and the human lysozyme gene (hLZ) was then inserted into the vector by a digestion-ligation method. The final vector containing the 8.5 kb mWAP 5′ promoter, 4.8 kb hLZ genomic DNA, and 8.0 kb mWAP 3′ genomic DNA was microinjected into pronuclei of fertilized mouse embryos, to successfully generate two transgenic mouse lines that expressed recombinant human lysozyme (rhLZ) in milk. The highest expression level of rhLZ was 0.45 g·L−1, and rhLZ exhibited the same antibacterial activity as native hLZ. Our results have provided a simple approach to construct a universal milk expression vector, and demonstrated that the resulting vector regulates the expression of hLZ in milk.
关键词: BAC recombinant methods gene expression human lysozyme transgenic mice milk expression vector
Intelligent methods for the process parameter determination of plastic injection molding
Huang GAO, Yun ZHANG, Xundao ZHOU, Dequn LI
《机械工程前沿(英文)》 2018年 第13卷 第1期 页码 85-95 doi: 10.1007/s11465-018-0491-0
Injection molding is one of the most widely used material processing methods in producing plastic products with complex geometries and high precision. The determination of process parameters is important in obtaining qualified products and maintaining product quality. This article reviews the recent studies and developments of the intelligent methods applied in the process parameter determination of injection molding. These intelligent methods are classified into three categories: Case-based reasoning methods, expert system-based methods, and data fitting and optimization methods. A framework of process parameter determination is proposed after comprehensive discussions. Finally, the conclusions and future research topics are discussed.
关键词: injection molding intelligent methods process parameters optimization
Temperature and structural responses of a single-section utility tunnel throughout fire exposure
Yanmin YANG; Ying XIONG; Yongqing LI; Xiangkun MENG; Peng WANG; Tianyuan CAI
《结构与土木工程前沿(英文)》 2022年 第16卷 第10期 页码 1351-1364 doi: 10.1007/s11709-022-0857-6
关键词: full lightweight concrete construction methods temperature response structural response fire test
《工程管理前沿(英文)》 2021年 第8卷 第4期 页码 503-518 doi: 10.1007/s42524-021-0163-3
关键词: Cyber–Physical Power System resilience assessment resilience optimization cascading failure modeling
From Green to Sustainability—Trends in the Assessment Methods of Green Buildings
Steve Hsueh-Ming Wang,Paula Williams,Jing Shi,Huojun Yang
《工程管理前沿(英文)》 2015年 第2卷 第2期 页码 114-121 doi: 10.15302/J-FEM-2015018
关键词: green sustainability buildings assessment methods rating systems
Edge preparation methods for cutting tools: a review
《机械工程前沿(英文)》 2023年 第18卷 第4期 doi: 10.1007/s11465-023-0766-y
关键词: edge preparation method preparation principle cutting edge geometry edge characterization tool performance
A review of measurement methods for peracetic acid (PAA)
Chen Cheng, Haodong Li, Jinling Wang, Hualin Wang, Xuejing Yang
《环境科学与工程前沿(英文)》 2020年 第14卷 第5期 doi: 10.1007/s11783-020-1266-5
关键词: Peroxyacetic acid Measurement methods Titration Colorimetric Chromatography NMR
null
《医学前沿(英文)》 2012年 第6卷 第3期 页码 311-316 doi: 10.1007/s11684-012-0205-7
In the current study, we assessed and evaluated the costs and benefits of three popular methods of general anesthesia practiced in our department for gynecological laparoscopic surgery in recent years. Sixty adult female patients who underwent elective gynecological laparoscopic surgery under general anesthesia were randomly divided into three groups: group V, group I and group C. In group V, anesthesia was induced intravenously with midazolam, remifentanil, propofol and vecuronium, and maintained with continuous infusion of propofol and remifentanil. In group I, anesthesia was intravenously induced with midazolam, fentanyl, propofol and vecuronium, and maintained with inhaled isoflurane and intravenous bonus of fentanyl. In group C, anesthesia was induced as in group I, but maintained with isoflurane inhalation combined with propofol-remifentanil infusion. All patients received vecuronium for muscle relaxation. Perioperative incidences of complications and total anesthesia costs for patients in all groups were recorded. In addition, postoperative satisfaction of the patients was also noted, and similar outcomes of the satisfaction were reported in all 60 patients. Although there was no statistical significance among groups, the incidence of postoperative nausea and vomiting were higher in group C, and the rates of shivering and the needs for analgesics were higher in group V. Anesthesia costs in group I were the lowest. Therefore, it is concluded that the costs of anesthesia induced with midazolam, fentanyl, propofol, vecuronium, and maintained with isoflurane, fentanyl and vecuronium are cheapest, and there is no significant difference in patients’ satisfaction and safety among the three above-mentioned methods of anesthesia in our department.
Behavioral methods for the functional assessment of hair cells in zebrafish
null
《医学前沿(英文)》 2017年 第11卷 第2期 页码 178-190 doi: 10.1007/s11684-017-0507-x
Zebrafish is an emerging animal model for studies on auditory system. This model presents high comparability with humans, good accessibility to the hearing organ, and high throughput capacity. To better utilize this animal model, methodologies need to be used to quantify the hearing function of the zebrafish. Zebrafish displays a series of innate and robust behavior related to its auditory function. Here, we reviewed the advantage of using zebrafish in auditory research and then introduced three behavioral tests, as follows: the startle response, the vestibular-ocular reflex, and rheotaxis. These tests are discussed in terms of their physiological characteristics, up-to-date technical development, and apparatus description. Test limitation and areas to improve are also introduced. Finally, we revealed the feasibility of these applications in zebrafish behavioral assessment and their potential in the high-throughput screening on hearing-related genes and drugs.
关键词: zebrafish (Danio rerio) behavior auditory startle response vestibular-ocular reflex rheotaxis
Computational methods for fracture in rock: a review and recent advances
Ali JENABIDEHKORDI
《结构与土木工程前沿(英文)》 2019年 第13卷 第2期 页码 273-287 doi: 10.1007/s11709-018-0459-5
关键词: numerical modelling method development rock mechanics fractured rock rock fracturing
标题 作者 时间 类型 操作
Analysis of effect of different construction methods of piles on the end effect on skin friction of piles
ZHOU Hongbo, CHEN Zhuchang
期刊论文
Performance of seven highway construction contracting methods analyzed by project size
Yuanxin ZHANG, Abdol CHINI, R. Edward MINCHIN Jr., Lourdes PTSCHELINZEW, Dev SHAH
期刊论文
毛志兵:发展新型建造方式 做强做大中国建造(2019年11月10日)
毛志兵(高级职称)
2021年01月20日
会议视频
Construction of a universal recombinant expression vector that regulates the expression of human lysozyme
Shen LIU, Shengzhe SHANG, Xuezhen YANG, Huihua ZHANG, Dan LU, Ning LI
期刊论文
Intelligent methods for the process parameter determination of plastic injection molding
Huang GAO, Yun ZHANG, Xundao ZHOU, Dequn LI
期刊论文
Temperature and structural responses of a single-section utility tunnel throughout fire exposure
Yanmin YANG; Ying XIONG; Yongqing LI; Xiangkun MENG; Peng WANG; Tianyuan CAI
期刊论文
Cyber–Physical Power System (CPPS): A review on measures and optimization methods of system resilience
期刊论文
From Green to Sustainability—Trends in the Assessment Methods of Green Buildings
Steve Hsueh-Ming Wang,Paula Williams,Jing Shi,Huojun Yang
期刊论文
A review of measurement methods for peracetic acid (PAA)
Chen Cheng, Haodong Li, Jinling Wang, Hualin Wang, Xuejing Yang
期刊论文
Comparative cost analysis of three different anesthesia methods in gynecological laparoscopic surgery
null
期刊论文